Luteolin inhibits NLRP3 inflammasome activation via blocking ASC oligomerization

The NLRP3 inflammasome is a caspase-1 containing multi-protein complex that controls the release of IL-1β and plays important roles in innate immune response. Since implicated pathogenesis variety diseases, it has become an increasingly interested target developing therapies for multiple diseases. We reported current study to determine how luteolin, natural phenolic compound found many vegetables medicinal herbs, would modulate both vivo vitro settings. First, we high-fat diet upregulated mRNA expression components Asc Casp1 adipose tissue ovariectomized mice, which were greatly reduced by dietary supplementation with luteolin. Of note, correlated levels Adgre1 encoding F4/80, established marker mature macrophages. also demonstrated luteolin inhibited inflammasome-derived activation secretion J774A.1 macrophages upon diverse stimuli including ATP, nigericin, or silica crystals. Luteolin step interfering ASC oligomerization. Taken together, these findings suggest may suppress induction process thus potentially be protective against NLRP3-mediated inflammatory